Crystal Structure of the Entire Ectodomain of gp130 INSIGHTS INTO THE MOLECULAR ASSEMBLY OF THE TALL CYTOKINE RECEPTOR COMPLEXES

Xu, YB; Kershaw, NJ; Luo, CS; Soo, P; Pocock, MJ; Czabotar, PE; Hilton, DJ; Nicola, NA; Garrett, TPJ; Zhang, JG

Author(s): Xu, YB; Kershaw, NJ; Luo, CS; Soo, P; Pocock, MJ; Czabotar, PE; Hilton, DJ; Nicola, NA; Garrett, TPJ; Zhang, JG;
Details: Publication Year 2010-07-09,Volume 285,Issue #28,Page 21214-21218
Journal Title: JOURNAL OF BIOLOGICAL CHEMISTRY
Publication Type: Journal Article
Abstract: gp130 is the shared signal-transducing receptor subunit for the large and important family of interleukin 6-like cytokines. Previous x-ray structures of ligand-receptor complexes of this family lack the three membrane-proximal domains that are essential for signal transduction. Here we report the crystal structure of the entire extracellular portion of human gp130 (domains 1-6, D1-D6) at 3.6 angstrom resolution, in an unliganded form, as well as a higher resolution structure of the membrane-proximal fibronectin type III domains (D4-D6) at 1.9 angstrom. This represents the first atomic resolution structure of the complete ectodomain of any "tall" cytokine receptor. These structures show that other than a reorientation of the D1 domain, there is little structural change in gp130 upon ligand binding. They also reveal that the interface between the D4 and D5 domains forms an acute bend in the gp130 structure. Key residues at this interface are highly conserved across the entire tall receptor family, suggesting that this acute bend may be a common feature of these receptors. Importantly, this geometry positions the C termini of the membrane-proximal fibronectin type III domains of the tall cytokine receptors in close proximity within the transmembrane complex, favorable for receptor-associated Janus kinases to trans-phosphorylate and activate each other.
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Keywords: PROXIMAL EXTRACELLULAR DOMAINS; SIGNAL TRANSDUCER GP130; INTEGRIN ALPHA-6-BETA-4; DIFFRACTION DATA; FIBRONECTIN; LIGAND; OLIGOMERIZATION; DIMERIZATION; RECOGNITION; ACTIVATION
Publisher's Version: https://doi.org/10.1074/jbc.C110.129502
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2010-07-09 12:00:00