Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription
- Author(s)
- Hulf, T; Sibbritt, T; Wiklund, ED; Bert, S; Strbenac, D; Statham, AL; Robinson, MD; Clark, SJ;
- Journal Title
- BMC GENOMICS
- Publication Type
- Journal Article
- Abstract
- Background: Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer. Results: Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells. Conclusions: We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression.
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- PROSTATE-CANCER; DNA METHYLATION; POSTTRANSCRIPTIONAL REGULATION; MICRORNA EXPRESSION; HUMAN GENOME; HYPERMETHYLATION; MIR-196B; CELLS; CHROMATIN; NETWORK
- Research Division(s)
- Bioinformatics
- Publisher's Version
- https://doi.org/10.1186/1471-2164-12-54
- Open Access at Publisher's Site
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037319/- Terms of Use/Rights Notice
- © 2011 Hulf et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creation Date: 2011-01-21 12:00:00