Gene deletion from Plasmodium falciparum using FLP and Cre recombinases: Implications for applied site-specific recombination
- Author(s)
- O'Neill, MT; Thuan, P; Healer, J; Richard, D; Cowman, AF;
- Details
- Publication Year 2011-01,Volume 41,Issue #1,Page 117-123
- Journal Title
- INTERNATIONAL JOURNAL FOR PARASITOLOGY
- Publication Type
- Journal Article
- Abstract
- The ability to manipulate the genome and induce site-specific recombination using either Flippase (FLP) or Cre recombinase has been useful in many systems including Plasmodium berghei for specific deletion events or to obtain conditional gene expression. To test whether these recombinases are active in Plasmodium falciparum we constructed gene knockouts that contain sequences recognised as templates for site-specific recombination. We tested the ability of FLP and Cre recombinases, expressed conditionally in P. falciparum, to mediate deletion of the human dihydrofolate reductase (hdhfr) drug resistance gene. We show that Cre recombinase is capable of efficient removal of hdhfr by site-specific recombination. In contrast, FLP recombinase is very inefficient, even at the optimum temperature of 30 C for this enzyme. These results demonstrate that Cre recombinase can be utilised in P. falciparum for deletion of specific sequences such as drug resistance genes. This can be exploited for recycling of drug resistance cassettes and for the design of specific recombination events in P. falciparum. (C) 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
- Publisher
- ELSEVIER SCI LTD
- Keywords
- CONDITIONAL MUTAGENESIS; PROTECTIVE IMMUNITY; MALARIA PARASITES; CELLS; CHROMOSOMES; VACCINE
- Publisher's Version
- https://doi.org/10.1016/j.ijpara.2010.08.001
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2011-01-01 12:00:00