Multipotential hematopoietic blast colony-forming cells exhibit delays in self-generation and lineage commitment
- Author(s)
- Metcalf, D; Ng, A; Mifsud, S; Di Rago, L;
- Details
- Publication Year 2010-09-14,Volume 107,Issue #37,Page 16257-16261
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Murine hematopoietic blast colony-forming cells (BL-CFCs) are able to generate up to 30,000 progeny blast cells within 10 d in agar cultures. Contained in these populations are large numbers of lineage-committed progenitor cells in the granulocytic and macrophage lineages. Sequential analyses of blast colonies revealed that self-generation of BL-CFCs occurs but is surprisingly late in clonal expansion, as is the emergence of progenitor cells committed to megakaryocytic and eosinophil lineages. Self-generating BL-CFCs were highly enriched in lineage(-) Kit(+) Sca1(+) CD34(-) Flt3R(-) populations, and colonies generated by such cells contained colony-forming units-spleen and formed erythroid and lymphoid progeny in vivo. The data suggest the existence of a hierarchical structure in BL-CFC populations with at least a subset being cells assayable as colony-forming units-spleen. Because BL-CFCs can self-generate and are able to generate lymphoid and myeloid populations, BL-CFCs appear to be ideal cells in which to analyze the processes of self-generation and lineage commitment in clonal in vitro cultures.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- colony-forming units–spleen ; megakaryocytes ; eosinophils
- Link To PubMed Central Version
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941309/
- Publisher's Version
- https://doi.org/10.1073/pnas.1011881107
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2010-09-14 12:00:00
Last Modified: 2014-12-23 12:11:55