A Complementary Role for the Tetraspanins CD37 and Tssc6 in Cellular Immunity

Gartlan, KH; Belz, GT; Tarrant, JM; Minigo, G; Katsara, M; Sheng, KC; Sofi, M; van Spriel, AB; Apostolopoulos, V; Plebanski, M; Robb, L; Wright, MD

Author(s): Gartlan, KH; Belz, GT; Tarrant, JM; Minigo, G; Katsara, M; Sheng, KC; Sofi, M; van Spriel, AB; Apostolopoulos, V; Plebanski, M; Robb, L; Wright, MD;
Details: Publication Year 2010-09-15,Volume 185,Issue #6,Page 3158-3166
Journal Title: JOURNAL OF IMMUNOLOGY
Publication Type: Journal Article
Abstract: The cooperative nature of tetraspanin-tetraspanin interactions in membrane organization suggests functional overlap is likely to be important in tetraspanin biology. Previous functional studies of the tetraspanins CD37 and Tssc6 in the immune system found that both CD37 and Tssc6 regulate T cell proliferative responses in vitro. CD37(-/-) mice also displayed a hyper-stimulatory dendritic cell phenotype and dysregulated humoral responses. In this study, we characterize "double knockout" mice (CD37(-/-) Tssc6(-/-)) generated to investigate functional overlap between these tetraspanins. Strong evidence for a cooperative role for these two proteins was identified in cellular immunity, where both in vitro T cell proliferative responses and dendritic cell stimulation capacity are significantly exaggerated in CD37(-/-) Tssc6(-/-) mice when compared with single knockout counterparts. Despite these exaggerated cellular responses in vitro, CD37(-/-) Tssc6(-/-) mice are not more susceptible to autoimmune induction. However, in vivo responses to pathogens appear poor in CD37(-/-) Tssc6(-/-) mice, which showed a reduced ability to produce influenza-specific T cells and displayed a rapid onset hyper-parasitemia when infected with Plasmodium yoelii. Therefore, in the absence of both CD37 and Tssc6, immune function is further altered when compared with CD37(-/-) or Tssc6(-/-) mice, demonstrating a complementary role for these two molecules in cellular immunity. The Journal of Immunology, 2010, 185: 3158-3166.
Publisher: AMER ASSOC IMMUNOLOGISTS
Keywords: SUPERFAMILY MEMBER CD151; MICE LACKING CD81; IN-VITRO; PROLIFERATIVE RESPONSES; CD9-DEFICIENT MICE; TRANSGENIC MICE; T-CELLS; CD9; MALARIA; EXPRESSION
Publisher's Version: https://doi.org/10.4049/jimmunol.0902867
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2010-09-15 12:00:00