Transience of MHC Class I-restricted antigen presentation after influenza A virus infection
- Author(s)
- Mintern, JD; Bedoui, S; Davey, GM; Moffat, JM; Doherty, PC; Turner, SJ;
- Details
- Publication Year 2009-04-21,Volume 106,Issue #16,Page 6724-6729
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Antigen expressed as MHC Class I glycoprotein (pMHCI) complexes on dendritic cells is the primary driver of CD8(+) T cell clonal expansion and differentiation. As we seek to define the molecular differences between acutely stimulated cytotoxic T lymphocyte (CTL) effectors and long-lived memory T cells, it is essential that we understand the duration of in vivo pMHCI persistence. Although infectious influenza A virus is readily cleared by mammalian hosts, that does not necessarily mean that all influenza antigen is totally eliminated. An exhaustive series of carefully controlled adoptive transfer experiments using 3 different carboxy fluorescein diacetate succinimidyl ester labeled T cell receptor-transgenic CTL populations and a spectrum of genetically engineered and wild-type influenza A viruses provided no evidence for pMHCI persistence over the 30-60-d interval after virus challenge. Molecular profiles identified in antigen-specific T cells at this time may thus be considered to reflect established immunologic memory and not recent CTL activation from a persistent pMHCI pool.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- CD8(+) T-CELLS; LYMPH-NODES; DENDRITIC CELLS; MEMORY; ACTIVATION; NAIVE; LUNG; DIFFERENTIATION; RESPONSES; CTL
- Publisher's Version
- https://doi.org/10.1073/pnas.0901128106
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2009-04-21 12:00:00