Generic construction of single component particles that elicit humoural and cellular immune responses without the need for adjuvants
- Author(s)
- White, PJ; Anastasopoulos, F; Church, JE; Kuo, CY; Boyd, BJ; Hickey, PLC; Tu, LS; Burns, P; Lew, AM; Heath, WR; Davey, GM; Pouton, CW;
- Details
- Publication Year 2008-12-09,Volume 26,Issue #52,Page 6824-6831
- Journal Title
- VACCINE
- Publication Type
- Journal Article
- Abstract
- Insoluble, pure protein particles could be advantageous as single-entity vaccines or as Carriers for small peptide epitopes. Dense gas anti-solvent precipitation was employed to produce pure protein particles which were found to be insoluble in water. As particulate and multimerized antigens are more immunogenic and hence more advantageous for vaccination, particles were produced via this method using ovalbumin as a model antigen. The particles produced had a mean diameter of similar to 300 nm, and remained as discrete particles at low pH. At neutral pH or in the presence of electrolyte, the Particles exhibited predictable flocculation behaviour to produce aggregates 1-5 mu m in diameter. Immunisation of mice with these flocculates elicited specific ovalbumin antibody production, T-cell proliferation and a cytotoxic T-cell response, all in the absence of adjuvant. Thus, dense gas processing could be used as a generic method to produce pure Protein particulate vaccines. (C) 2008 Elsevier Ltd. All rights reserved.
- Publisher
- ELSEVIER SCI LTD
- Keywords
- SUPERCRITICAL CARBON-DIOXIDE; VIRUS-LIKE PARTICLES; VACCINE FORMULATION; T-CELL; POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES; IN-VIVO; NANOPARTICLES; OVALBUMIN; SIZE; IMMUNOGENICITY
- Publisher's Version
- https://doi.org/10.1016/j.vaccine.2008.09.087
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2008-12-09 12:00:00