Distinct functional capacities of mouse thymic and splenic dendritic cell populations
Details
Publication Year 2008-11,Volume 86,Issue #8,Page 700-708
Journal Title
IMMUNOLOGY AND CELL BIOLOGY
Publication Type
Journal Article
Abstract
Dendritic cells (DC) are antigen-presenting cells that activate naive T cells. Murine DC are a heterogeneous population and can be subdivided into distinct subsets with different immune regulatory functions, namely the conventional DC (cDC), which include the CD8(+)Sirp alpha(-) and CD8(-)Sirp alpha(+) DC, and the plasmacytoid DC (pDC). In this study, the phenotype and function of DC subsets in both the thymus and spleen were compared. Significant differences between the thymic and splenic DC were observed in the expression of genes encoding chemokine receptors (CCRs), toll-like receptors (TLRs) and chemokines. Thymic DC expressed high levels of genes encoding a unique set of chemokines (CCL17 and CCL22) known to be important for T-cell development. Moreover, the capacity of the DC from the two organs to produce IL-6, IFN-alpha and IL-12p70 in response to the TLR 9 agonist CpG differed markedly, indicating intrinsic functional differences between subsets with similar surface phenotype. These results indicate that the microenvironment is an important factor that contributes to the functional specification of DC subsets in different lymphoid tissues.
Publisher
NATURE PUBLISHING GROUP
Keywords
CD4 T-CELLS; CUTTING EDGE; IN-VIVO; ANTIGEN PRESENTATION; CROSS-PRESENTATION; LYMPH-NODES; DIFFERENTIAL PRODUCTION; SURFACE PHENOTYPE; IMMUNE-RESPONSE; CLONAL DELETION
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Creation Date: 2008-11-01 12:00:00
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