A key role for G-CSF-induced neutrophil production and trafficking during inflammatory arthritis

Eyles, JL; Hickey, MJ; Norman, MU; Croker, BA; Roberts, AW; Drake, SF; James, WG; Metcalf, D; Campbell, IK; Wicks, IP

Author(s): Eyles, JL; Hickey, MJ; Norman, MU; Croker, BA; Roberts, AW; Drake, SF; James, WG; Metcalf, D; Campbell, IK; Wicks, IP;
Details: Publication Year 2008-12-15,Volume 112,Issue #13,Page 5193-5201
Journal Title: BLOOD
Publication Type: Journal Article
Abstract: We have previously shown that G-CSF-deficient (G-CSF(-/-)) mice are markedly protected from collagen-induced arthritis ( CIA), which is the major murine model of rheumatoid arthritis, and now investigate the mechanisms by which G-CSF can promote inflammatory disease. Serum G-CSF levels were significantly elevated during CIA. Reciprocal bone marrow chimeras using G-CSF(-/-), G-CSFR(-/-), and wild-type (WT) mice identified nonhematopoietic cells as the major producers of G-CSF and hematopoietic cells as the major responders to G-CSF during CIA. Protection against CIA was associated with relative neutropenia. Depletion of neutrophils or blockade of the neutrophil adhesion molecule, Mac-1, dramatically attenuated the progression of established CIA in WT mice. Intravital microscopy of the microcirculation showed that both local and systemic administration of G-CSF significantly increased leukocyte trafficking into tissues in vivo. G-CSF induced trafficking was Mac-1 dependent, and G-CSF up-regulated CD11b expression on neutrophils. Multiphoton microscopy of synovial vessels in the knee joint during CIA revealed significantly fewer adherent Gr-1(+) neutrophils in G-CSF(-/-) mice compared with WT mice. These data confirm a central proinflammatory role for G-CSF in the pathogenesis of inflammatory arthritis, which may be due to the promotion of neutrophil trafficking into inflamed joints, in addition to G-CSF induced neutrophil production. (Blood. 2008; 112: 5193-5201)
Publisher: AMER SOC HEMATOLOGY
Keywords: COLONY-STIMULATING FACTOR
Publisher's Version: https://doi.org/10.1182/blood-2008-02-139535
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2008-12-15 12:00:00

Last Modified: 2014-12-23 01:14:04