Secretome-Based Proteomic Profiling of Ras-Transformed MDCK Cells Reveals Extracellular Modulators of Epithelial-Mesenchymal Transition
Publication Year 2009-06, Volume 8, Issue #6, Page 2827-2837
- Journal Title
- JOURNAL OF PROTEOME RESEARCH
- Publication Type
- Journal Article
- Epithelial-mesenchymal transition (EMT) is a highly conserved morphogenetic process by which epithelial cells lose their basic morphological characteristics such as cell-cell contact and gain mesenchymal properties such as increased motility and invasiveness. To gain insights into proteins released from cells that modulate the EMT process, we compared secretome protein expression profiles of MDCK cells and Ras-transformed MDCK cells (21D1) that stably express oncogenic Ras using 2D-DIGE/LC-MS/MS. Differentially expressed secretome proteins were compared with their corresponding gene expression profiles using the Affymetrix GeneChip system. Down-regulated proteins were predominantly involved with cell-cell contact and cell-matrix adhesion (e.g., desmocollin 2, clusterin, collagen XVII and transforming growth factor-beta induced protein ig-h3), while up-regulated proteins were proteases and factors that promote migration (MMP-1, kallikrein 6, TIMP-1, and S100A4/metastasin). Many of the secretome proteins identified in this study have not been previously identified in the context of EMT and may shed light on the underlying mechanisms associated with this cellular process.
- AMER CHEMICAL SOC
- E-CADHERIN EXPRESSION; LUNG-CANCER CELLS; TISSUE INHIBITOR; TRANSCRIPTION FACTOR; TUMOR PROGRESSION; MATRIX METALLOPROTEINASES; PROTEIN S100A4; SCATTER FACTOR; GROWTH-FACTOR; COLON-CANCER
- Publisher's Version
- Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2009-06-01 12:00:00Last Modified: 0001-01-01 12:00:00