Plasmodium falciparum merozoite surface protein 2 is unstructured and forms amyloid-like fibrils

Adda, CG; Murphy, VJ; Sunde, M; Waddington, LJ; Schloegel, J; Talbo, GH; Vingas, K; Kienzle, V; Masciantonio, R; Howlett, GJ; Hodder, AN; Foley, M; Anders, RF

Author(s): Adda, CG; Murphy, VJ; Sunde, M; Waddington, LJ; Schloegel, J; Talbo, GH; Vingas, K; Kienzle, V; Masciantonio, R; Howlett, GJ; Hodder, AN; Foley, M; Anders, RF;
Details: Publication Year 2009-08,Volume 166,Issue #2,Page 159-171
Journal Title: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Publication Type: Journal Article
Abstract: Several merozoite surface proteins are being assessed as potential components of a vaccine against Plasmodium falciparum, the cause of the most serious form of human malaria. One of these proteins, merozoite surface protein 2 (MSP2), is unusually hydrophilic and contains tandem sequence repeats, characteristics of intrinsically unstructured proteins. A range of physicochemical studies has confirmed that recombinant forms of MSP2 are largely unstructured. Both dimorphic types of MSP2 (3D7 and FC27) are equivalently extended in solution and form amyloid-like fibrils although with different kinetics and structural characteristics. These fibrils have a regular underlying P-sheet structure and both fibril types stain with Congo Red, but only the FC27 fibrils stain with Thioflavin T. 3D7 MSP2 fibrils seeded the growth of fibrils from 3D7 or FC27 MSP2 monomer indicating the involvement of a conserved region of MSP2 in fibril formation. Consistent with this, digestion of fibrils with proteinase K generated resistant peptides, which included the N-terminal conserved region of MSP2. A monoclonal antibody that reacted preferentially with monomeric recombinant MSP2 did not react with the antigen in situ on the merozoite surface. Glutaraldehyde cross-linking of infected erythrocytes generated MSP2 oligomers similar to those formed by polymeric recombinant MSP2. We conclude that MSP2 oligomers containing intermolecular P-strand interactions similar to those in amyloid fibrils may be a component of the fibrillar surface coat on P. falciparum merozoites. (C) 2009 Elsevier B.V. All rights reserved.
Publisher: ELSEVIER SCIENCE BV
Keywords: APICAL MEMBRANE ANTIGEN-1; BLOOD-STAGE VACCINE; MALARIA VACCINE; PRION PROTEIN; S-ANTIGEN; STRUCTURAL DIVERSITY; ESCHERICHIA-COLI; ALPHA-SYNUCLEIN; TERMINAL DOMAIN; CLINICAL-TRIAL
Publisher's Version: https://doi.org/10.1016/j.molbiopara.2009.03.012
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Creation Date: 2009-08-01 12:00:00