Monoclonal antibodies generated by DNA immunization recognize CD2 from a broad range of primates
- Author(s)
- Brady, JL; Mannering, SI; Kireta, S; Coates, PT; Proietto, AI; Cowan, PJ; d'Apice, AJF; Lew, AM;
- Details
- Publication Year 2009-07,Volume 87,Issue #5,Page 413-418
- Journal Title
- IMMUNOLOGY AND CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- Using heterologous prime-boost (DNA immunization followed by immunization with transfected cells), we have generated depleting mouse anti-baboon CD2 monoclonal antibodies (mAb). These anti-CD2 mAb recognized a diverse range of primate CD2 from New World monkeys and Old World monkeys to humans and have potent immunosuppressive activity for human allo-MLR responses and anti-tetanus-toxoid recall responses. There was no upregulation of activation markers or release of cytokines when the mAb were incubated with human peripheral blood mononuclear cells. Using chimeric NOD-SCID IL2r gamma(null) mice, the mAb were shown to deplete human and cynomolgus monkey T cells in vivo. These anti-CD2 mAb may therefore be important immunological tools in allo-and xenotransplantation. Immunology and Cell Biology (2009) 87, 413-418; doi:10.1038/icb.2009.4; published online 10 February 2009
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- HEMATOPOIETIC-CELL TRANSPLANTATION; HUMANIZED ANTI-CD2 MAB; HUMAN T-CELLS; ERYTHROCYTE RECEPTOR; CYTOKINE-RELEASE; IN-VITRO; LYMPHOCYTE; ACTIVATION; RECIPIENTS; ALLOGRAFT
- Publisher's Version
- https://doi.org/10.1038/icb.2009.4
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2009-07-01 12:00:00