Bcl-2 family member Bcl-G is not a proapoptotic protein
Journal Title
CELL DEATH & DISEASE
Publication Type
Journal Article
Abstract
The three major subgroups of the Bcl-2 family, including the prosurvival Bcl-2-like proteins, the proapoptotic Bcl-2 homology (BH) 3-only proteins and Bax/Bak proteins, regulate the mitochondrial apoptotic pathway. In addition, some outliers within the Bcl-2 family do not fit into these subgroups. One of them, Bcl-G, has a BH2 and a BH3 region, and was proposed to trigger apoptosis. To investigate the physiological role of Bcl-G, we have inactivated the gene in the mouse and generated monoclonal antibodies to determine its expression. Although two isoforms of Bcl-G exist in human, only one is found in mice. mBcl-G is expressed in a range of epithelial as well as in dendritic cells. Loss of Bcl-G did not appear to affect any of these cell types. mBcl-G only binds weakly to prosurvival members of the Bcl-2 family, and in a manner that is independent of its BH3 domain. To understand what the physiological role of Bcl-G might be, we searched for Bcl-G-binding partners through immunoprecipitation/mass spectroscopy and yeast-two-hybrid screening. Although we did not uncover any Bcl-2 family member in these screens, we found that Bcl-G interacts specifically with proteins of the transport particle protein complex. We conclude that Bcl-G most probably does not function in the classical stress-induced apoptosis pathway, but rather has a role in protein trafficking inside the cell. Cell Death and Disease (2012) 3, e404; doi:10.1038/cddis.2012.130; published online 11 October 2012
Publisher
NATURE PUBLISHING GROUP
Keywords
PRO-APOPTOTIC MEMBER; ACUTE LYMPHOBLASTIC-LEUKEMIA; PROGRAMMED CELL-DEATH; DENDRITIC CELLS; BH3-ONLY PROTEINS; GENE-EXPRESSION; LIFE-SPAN; IN-VIVO; LIGANDS; CANCER
WEHI Research Division(s)
Molecular Genetics Of Cancer
Rights Notice
Copyright © 2012 Macmillan Publishers Limited This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/


Creation Date: 2012-10-01 12:00:00
Last Modified: 0001-01-01 12:00:00
An error has occurred. This application may no longer respond until reloaded. Reload 🗙