Isolation of Mouse Mammary Epithelial Subpopulations: A Comparison of Leading Methods
- Author(s)
- Smalley, MJ; Kendrick, H; Sheridan, JM; Regan, JL; Prater, MD; Lindeman, GJ; Watson, CJ; Visvader, JE; Stingl, J;
- Details
- Publication Year 2012-06,Volume 17,Issue #2,Page 91-97
- Journal Title
- JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
- Publication Type
- Journal Article
- Abstract
- Isolation of mammary epithelial subpopulations, including stem and progenitor cells, has become a standard technique in recent years. However, a number of methods and approaches for this have developed and the relative benefits of the different approaches, and the reason for their development, have not always been clear. Here, three of the leading laboratories working on the separation of mammary cell subpopulations have summarised their methods, highlighted their differences and similarities and also discussed the reasoning behind the approaches they have taken. This article will assist workers establishing mammary cell separation protocols in their laboratories to make informed choices about the methods they should use.
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- STEM-CELLS; GLAND; DIFFERENTIATION; EXPRESSION
- Research Division(s)
- Stem Cells And Cancer
- Publisher's Version
- https://doi.org/10.1007/s10911-012-9257-1
- Terms of Use/Rights Notice
- © Springer, Part of Springer Science+Business Media
Creation Date: 2012-06-01 12:00:00