In vivo excitation of nanoparticles using luminescent bacteria
- Author(s)
- Dragavon, J; Blazquez, S; Rekiki, A; Samson, C; Theodorou, I; Rogers, KL; Tournebize, R; Shorte, SL;
- Details
- Publication Year 2012-06-05,Volume 109,Issue #23,Page 8890-8895
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- The lux operon derived from Photorhabdus luminescens incorporated into bacterial genomes, elicits the production of biological chemiluminescence typically centered on 490 nm. The light-producing bacteria are widely used for in vivo bioluminescence imaging. However, in living samples, a common difficulty is the presence of blue-green absorbers such as hemoglobin. Here we report a characterization of fluorescence by unbound excitation from luminescence, a phenomenon that exploits radiating luminescence to excite nearby fluorophores by epifluorescence. We show that photons from bioluminescent bacteria radiate over mesoscopic distances and induce a red-shifted fluorescent emission from appropriate fluorophores in a manner distinct from bioluminescence resonance energy transfer. Our results characterizing fluorescence by unbound excitation from luminescence, both in vitro and in vivo, demonstrate how the resulting blue-to-red wavelength shift is both necessary and sufficient to yield contrast enhancement revealing mesoscopic proximity of luminescent and fluorescent probes in the context of living biological tissues.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- REPORTER-GENE-EXPRESSION; ENERGY-TRANSFER; BIOLUMINESCENCE; LUCIFERASE; MICROSCOPY; BIOLOGY; FRET; BRET
- Research Division(s)
- Systems Biology And Personalised Medicine
- Link To PubMed Central Version
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384150/
- Publisher's Version
- https://doi.org/10.1073/pnas.1204516109
- Terms of Use/Rights Notice
- Copyright © 2013 National Academy of Sciences.
Creation Date: 2012-06-05 12:00:00