Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies
- Author(s)
- Giam, M; Mintern, JD; Rautureau, GJP; Hinds, MG; Strasser, A; Bouillet, P;
- Journal Title
- CELL DEATH & DISEASE
- Publication Type
- Journal Article
- Abstract
- Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8(+) conventional dendritic cells (DCs) in hematopoietic tissues and diverse epithelial cell types, including those lining the gastrointestinal and respiratory tracts. The Bcl-G monoclonal antibodies represent new tools for studying this protein, using a variety of techniques, including immunoprecipitation and flow cytometry. Cell Death and Disease (2012) 3, e378; doi: 10.1038/cddis.2012.117; published online 23 August 2012
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- PRO-APOPTOTIC MEMBER; CELL-DEATH; EXPRESSION; PROTEIN; CANCER; MELK; FAU
- Research Division(s)
- Molecular Genetics Of Cancer
- Link To PubMed Central Version
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434660/
- Publisher's Version
- https://doi.org/10.1038/cddis.2012.117
- Open Access at Publisher's Site
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434660/- Terms of Use/Rights Notice
- Copyright © 2012 Macmillan Publishers Limited This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
Creation Date: 2012-08-01 12:00:00