Ultramarine, a Chromoprotein Acceptor for Forster Resonance Energy Transfer
- Author(s)
- Pettikiriarachchi, A; Gong, L; Perugini, MA; Devenish, RJ; Prescott, M;
- Details
- Publication Year 2012-07-16,Volume 7,Issue #7,Page e41028
- Journal Title
- PLOS ONE
- Publication Type
- Journal Article
- Abstract
- We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; epsilon (585) (nm), 64,000 M-1. cm(-1)) for use as a Forster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
- Publisher
- PUBLIC LIBRARY SCIENCE
- Keywords
- RED FLUORESCENT PROTEIN; POCILLOPORIN PIGMENT; CRYSTAL-STRUCTURE; CELL-DEATH; BIOSENSORS; GFP; DYNAMICS; FRET
- Research Division(s)
- Infection And Immunity
- Publisher's Version
- https://doi.org/10.1371/journal.pone.0041028
- Open Access at Publisher's Site
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397996/- Terms of Use/Rights Notice
- Copyright: © 2012 Pettikiriarachchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creation Date: 2012-07-16 12:00:00