Ultramarine, a Chromoprotein Acceptor for Forster Resonance Energy Transfer
Details
Publication Year 2012-07-16, Volume 7, Issue #7, Page e41028
Journal Title
PLOS ONE
Publication Type
Journal Article
Abstract
We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; epsilon (585) (nm), 64,000 M-1. cm(-1)) for use as a Forster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
Publisher
PUBLIC LIBRARY SCIENCE
Keywords
RED FLUORESCENT PROTEIN; POCILLOPORIN PIGMENT; CRYSTAL-STRUCTURE; CELL-DEATH; BIOSENSORS; GFP; DYNAMICS; FRET
WEHI Research Division(s)
Infection And Immunity
Rights Notice
Copyright: © 2012 Pettikiriarachchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Creation Date: 2012-07-16 12:00:00
Last Modified: 0001-01-01 12:00:00
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