Concordant mast cell and basophil production by individual hematopoietic blast colony-forming cells
Details
Publication Year 2013-05-28, Volume 110, Issue #22, Page 9031-9035
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type
Journal Article
Abstract
Previous studies have shown that mouse bone marrow cells can produce mast cells when stimulated in vitro by stem cell factor (SCF) and interleukin-3 (IL-3). Experiments to define the marrow cells able to generate mast cells showed that the most active subpopulations were the Kit(+) Sca1(-) progenitor cell fraction and the more ancestral Kit(+) Sca1(+) blast colony-forming cell fraction. In clonal cultures, up to 64% of blast colony-forming cells were able to generate mast cells when stimulated by SCF and IL-3, and, of these, the most active were those in the CD34(-) Flt3R(-) long-term repopulating cell fraction. Basophils, identified by the monoclonal antibody mMCP-8 to mouse mast cell serine protease-8, were also produced by 50% of blast colony-forming cells with a strong concordance in the production of both cell types by individual blast colony-forming cells. Enriched populations of marrow-derived basophils were shown to generate variable numbers of mast cells after a further incubation with SCF and IL-3. The data extend the repertoire of lineage-committed cells able to be produced by multipotential hematopoietic blast colony-forming cells and show that basophils and mast cells can have common ancestral cells and that basophils can probably generate mast cells at least under defined in vitro conditions.
Publisher
NATL ACAD SCIENCES
Keywords
GROWTH-FACTOR; MOUSE BASOPHILS; IN-VITRO; INTERLEUKIN-3; PURIFICATION; MICE; DIFFERENTIATION; EXPRESSION; IMMUNITY; MARKER
WEHI Research Division(s)
Cancer And Haematology
NHMRC Grants
NHMRC/461219 NHMRC/1016647
Rights Notice
Copyright © 2013 National Academy of Sciences.


Creation Date: 2013-05-28 12:00:00
Last Modified: 0001-01-01 12:00:00
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