Concordant mast cell and basophil production by individual hematopoietic blast colony-forming cells
- Author(s)
- Metcalf, D; Ng, AP; Baldwin, TM; Di Rago, L; Mifsud, S;
- Details
- Publication Year 2013-05-28,Volume 110,Issue #22,Page 9031-9035
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Previous studies have shown that mouse bone marrow cells can produce mast cells when stimulated in vitro by stem cell factor (SCF) and interleukin-3 (IL-3). Experiments to define the marrow cells able to generate mast cells showed that the most active subpopulations were the Kit(+) Sca1(-) progenitor cell fraction and the more ancestral Kit(+) Sca1(+) blast colony-forming cell fraction. In clonal cultures, up to 64% of blast colony-forming cells were able to generate mast cells when stimulated by SCF and IL-3, and, of these, the most active were those in the CD34(-) Flt3R(-) long-term repopulating cell fraction. Basophils, identified by the monoclonal antibody mMCP-8 to mouse mast cell serine protease-8, were also produced by 50% of blast colony-forming cells with a strong concordance in the production of both cell types by individual blast colony-forming cells. Enriched populations of marrow-derived basophils were shown to generate variable numbers of mast cells after a further incubation with SCF and IL-3. The data extend the repertoire of lineage-committed cells able to be produced by multipotential hematopoietic blast colony-forming cells and show that basophils and mast cells can have common ancestral cells and that basophils can probably generate mast cells at least under defined in vitro conditions.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- GROWTH-FACTOR; MOUSE BASOPHILS; IN-VITRO; INTERLEUKIN-3; PURIFICATION; MICE; DIFFERENTIATION; EXPRESSION; IMMUNITY; MARKER
- Research Division(s)
- Cancer And Haematology
- Link To PubMed Central Version
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3670339/
- Publisher's Version
- https://doi.org/10.1073/pnas.1307711110
- NHMRC Grants
- NHMRC/461219, NHMRC/1016647,
- Terms of Use/Rights Notice
- Copyright © 2013 National Academy of Sciences.
Creation Date: 2013-05-28 12:00:00