Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity
Journal Title
NATURE COMMUNICATIONS
Publication Type
Journal Article
Abstract
The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.
Publisher
NATURE PUBLISHING GROUP
Keywords
T-LYMPHOCYTE DIVISION; PLASMA-CELL; B-CELLS; TRANSCRIPTION FACTOR; SELF-RENEWAL; PAR COMPLEX; DIFFERENTIATION; SEGREGATION; ANTIGEN; EXPRESSION
WEHI Research Division(s)
Molecular Immunology; Immunology
Rights Notice
© 2013 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.


Creation Date: 2013-04-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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