Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity
- Author(s)
- Hawkins, ED; Oliaro, J; Kallies, A; Belz, GT; Filby, A; Hogan, T; Haynes, N; Ramsbottom, KM; Van Ham, V; Kinwell, T; Seddon, B; Davies, D; Tarlinton, D; Lew, AM; Humbert, PO; Russell, SM;
- Journal Title
- NATURE COMMUNICATIONS
- Publication Type
- Journal Article
- Abstract
- The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- T-LYMPHOCYTE DIVISION; PLASMA-CELL; B-CELLS; TRANSCRIPTION FACTOR; SELF-RENEWAL; PAR COMPLEX; DIFFERENTIATION; SEGREGATION; ANTIGEN; EXPRESSION
- Research Division(s)
- Molecular Immunology; Immunology
- Publisher's Version
- https://doi.org/10.1038/ncomms2796
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Creation Date: 2013-04-01 12:00:00