Convergent differentiation: myeloid and lymphoid pathways to murine plasmacytoid dendritic cells
- Author(s)
- Sathe, P; Vremec, D; Wu, L; Corcoran, L; Shortman, K;
- Details
- Publication Year 2013-01-03,Volume 121,Issue #1,Page 11-19
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- The developmental origin of IFN-producing plasmacytoid dendritic cells (pDCs) has been uncertain. In the present study, we tracked the development of pDCs in cultures of BM precursors stimulated with Flt3 ligand. Common myeloid precursors (CMPs) produced both conventional DCs (cDCs) and pDCs via the DC-restricted common DC precursor. Common lymphoid precursors (CLPs) produced only a few cDCs with variable efficiency, but produced pDCs via a transient intermediate precursor with B-cell potential. The pDCs of both origins produced IFN-alpha when stimulated with CpG oligonucleotides. The pDCs of CLP origin showed evidence of past RAG1 expression and had D-J rearrangements in IgH genes. Most pDCs and all cDCs of CMP origin lacked these signs of a lymphoid past. However, in these cultures, some pDCs of CMP origin showed evidence of past RAG1 expression and had D-J IgH gene rearrangements; most of these derived from a subset of CMPs already expressing RAG1. (Blood. 2013; 121(1):11-19)
- Publisher
- AMER SOC HEMATOLOGY
- Keywords
- TRANSCRIPTION FACTOR E2-2; BONE-MARROW CULTURES; CUTTING EDGE; IN-VITRO; PROGENITORS; PRECURSORS; IDENTIFICATION; ACTIVATION; GENERATION; SUBSETS
- Research Division(s)
- Molecular Immunology; Immunology
- Publisher's Version
- https://doi.org/10.1182/blood-2012-02-413336
- Terms of Use/Rights Notice
- © 2013 by The American Society of Hematology
Creation Date: 2013-01-03 12:00:00