NK cells and conventional dendritic cells engage in reciprocal activation for the induction of inflammatory responses during Plasmodium berghei ANKA infection
- Author(s)
- Ryg-Cornejo, V; Nie, CQ; Bernard, NJ; Lundie, RJ; Evans, KJ; Crabb, BS; Schofield, L; Hansen, DS;
- Details
- Publication Year 2013-02,Volume 218,Issue #2,Page 263-271
- Journal Title
- IMMUNOBIOLOGY
- Publication Type
- Journal Article
- Abstract
- Cerebral malaria (CM) is the most severe syndrome associated with Plasmodium falciparum infections. Experimental evidence suggests that disease results from the sequestration of parasitized-red blood cells (pRBCs) together with inflammatory leukocytes within brain capillaries. We have previously shown that NK cells stimulate migration of CXCR3(+) T cells to the brain of Plasmodium berghei ANKA-infected mice. Here we investigated whether interactions between NK cells and dendritic cells (DCs) are required for the induction of T cell responses involved in disease. For that, NK cell-depleted and control mice were infected with transgenic parasites expressing model T cell epitopes. T cells from TCR transgenic mice specific for those epitopes were adoptively transferred and proliferation was determined. NK cell depletion significantly reduced CD8(+) but not CD4(+) DC-mediated T cell priming. Lack of NK cells did not compromise CD8(+) T cell responses in IL-12(-/-) mice, suggesting that NK cells stimulate IL-12 output by DCs required for optimal T cell priming. The contribution of DCs to NK cell function was also investigated. DC depletion and genetic deletion of IL-12 dramatically reduced NK cell-mediated IFN-gamma responses to malaria. Thus NK cells and DCs engage in reciprocal activation for the induction of inflammatory responses involved in severe malaria. (C) 2012 Elsevier GmbH. All rights reserved.
- Publisher
- ELSEVIER GMBH, URBAN & FISCHER VERLAG
- Keywords
- MURINE CEREBRAL MALARIA; TUMOR-NECROSIS-FACTOR; NATURAL-KILLER-CELLS; FALCIPARUM-MALARIA; T-CELLS; INTERFERON-GAMMA; GENE-COMPLEX; CROSS-TALK; IFN-GAMMA; PATHOGENESIS
- Research Division(s)
- Infection And Immunity
- Publisher's Version
- https://doi.org/10.1016/j.imbio.2012.05.018
- Terms of Use/Rights Notice
- Copyright © 2012 Elsevier GmbH. All rights reserved.
Creation Date: 2013-02-01 12:00:00