Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation
- Author(s)
- Moffat, JM; Segura, E; Khoury, G; Caminschi, I; Cameron, PU; Lewin, SR; Villadangos, JA; Mintern, JD;
- Details
- Publication Year 2013-03,Volume 43,Issue #3,Page 595-605
- Journal Title
- EUROPEAN JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Bone marrow stromal cell-2 (BST-2) has major roles in viral tethering and modulation of interferon production. Here we investigate BST-2 as a receptor for the delivery of antigen to dendritic cells (DCs). We show that BST-2 is expressed by a panel of mouse and human DC subsets, particularly under inflammatory conditions. The outcome of delivering antigen to BST-2 expressed by steady state and activated plasmacytoid DC (pDC) or conventional CD8+ and CD8 DCs was determined. T-cell responses were measured for both MHC class I (MHCI) and MHC class II (MHCII) antigen presentation pathways in vitro. Delivering antigen via BST-2 was compared with that via receptors DEC205 or Siglec-H. We show that despite a higher antigen load and faster receptor internalisation, when antigen is delivered to steady state or activated pDC via BST-2, BST-2-targeted activated conventional DCs present antigen more efficiently. Relative to DEC205, BST-2 was inferior in its capacity to deliver antigen to the MHCI cross-presentation pathway. In contrast, BST-2 was superior to Siglec-H at initiating either MHCI or MHCII antigen presentation. In summary, BST-2 is a useful receptor to target with antigen, given its broad expression pattern and ability to access both MHCI and MHCII presentation pathways with relative efficiency.
- Publisher
- WILEY-BLACKWELL
- Keywords
- Antigen-presenting cells;Antigen presentation/processing;Dendritic cells;Vaccination
- Research Division(s)
- Immunology
- Publisher's Version
- https://doi.org/10.1002/eji.201242799
- Terms of Use/Rights Notice
- © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Creation Date: 2013-03-01 12:00:00