Thymic but not splenic CD8(+) DCs can efficiently cross-prime T cells in the absence of licensing factors
Details
Publication Year 2011-09, Volume 41, Issue #9, Page 2544-2555
Journal Title
EUROPEAN JOURNAL OF IMMUNOLOGY
Publication Type
Journal Article
Abstract
Cross-presentation is an important mechanism to elicit both immune defenses and tolerance. Although only a few DC subsets possess the machinery required for cross-presentation, little is known about differences in cross-presenting capabilities of DCs belonging to the same subpopulation but localized in different lymphoid organs. In this study, we demonstrate that steady-state thymic CD8(+) DCs can efficiently cross-prime naive CD8(+) T cells in the absence of costimulation. Surprisingly, cross-priming by splenic CD8(+) DCs was dependent on licensing factors such as GM-CSF. In the absence of GM-CSF, antigen-MHC-class-I complexes were detected on thymic but not on splenic CD8(+) DCs, indicating that the cross-presentation capacity of the thymic subpopulation was higher. The observed cross-priming differences between thymic and splenic CD8(+) DCs did not correlate with differential antigen capture or costimulatory molecules found on the surface of DCs. Moreover, we did not detect overall impairment of antigen presentation, as peptide-loaded splenic CD8(+) DCs were able to induce CD8(+) T-cell proliferation. The observation that thymic CD8(+) DCs are more efficient than splenic CD8(+) DCs in T-cell cross-priming in the absence of licensing factors indicates that the requirements for efficient antigen presentation differ between these cells.
Publisher
WILEY-BLACKWELL
Keywords
CD8-ALPHA(+) DENDRITIC CELLS; IN-VIVO; STEADY-STATE; ANTIGEN; TOLERANCE; RECEPTOR; SUBSET; IMMUNITY; CD103(+); MICROENVIRONMENT
Rights Notice
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Creation Date: 2011-09-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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