A Phase 1 Trial of MSP2-C1, a Blood-Stage Malaria Vaccine Containing 2 Isoforms of MSP2 Formulated with Montanide (R) ISA 720

McCarthy, JS; Marjason, J; Elliott, S; Fahey, P; Bang, G; Malkin, E; Tierney, E; Aked-Hurditch, H; Adda, C; Cross, N; Richards, JS; Fowkes, FJI; Boyle, MJ; Long, C; Druilhe, P; Beeson, JG; Anders, RF

Author(s): McCarthy, JS; Marjason, J; Elliott, S; Fahey, P; Bang, G; Malkin, E; Tierney, E; Aked-Hurditch, H; Adda, C; Cross, N; Richards, JS; Fowkes, FJI; Boyle, MJ; Long, C; Druilhe, P; Beeson, JG; Anders, RF;
Details: Publication Year 2011-09-19,Volume 6,Issue #9,Page -
Journal Title: PLOS ONE
Publication Type: Journal Article
Abstract: Background: In a previous Phase 1/2b malaria vaccine trial testing the 3D7 isoform of the malaria vaccine candidate Merozoite surface protein 2 (MSP2), parasite densities in children were reduced by 62%. However, breakthrough parasitemias were disproportionately of the alternate dimorphic form of MSP2, the FC27 genotype. We therefore undertook a dose-escalating, double-blinded, placebo-controlled Phase 1 trial in healthy, malaria-naive adults of MSP2-C1, a vaccine containing recombinant forms of the two families of msp2 alleles, 3D7 and FC27 (EcMSP2-3D7 and EcMSP2-FC27), formulated in equal amounts with Montanide (R) ISA 720 as a water-in-oil emulsion. Methodology/Principal Findings: The trial was designed to include three dose cohorts (10, 40, and 80 mu g), each with twelve subjects receiving the vaccine and three control subjects receiving Montanide (R) ISA 720 adjuvant emulsion alone, in a schedule of three doses at 12-week intervals. Due to unexpected local reactogenicity and concern regarding vaccine stability, the trial was terminated after the second immunisation of the cohort receiving the 40 mu g dose; no subjects received the 80 mu g dose. Immunization induced significant IgG responses to both isoforms of MSP2 in the 10 mu g and 40 mu g dose cohorts, with antibody levels by ELISA higher in the 40 mu g cohort. Vaccine-induced antibodies recognised native protein by Western blots of parasite protein extracts and by immunofluorescence microscopy. Although the induced anti-MSP2 antibodies did not directly inhibit parasite growth in vitro, IgG from the majority of individuals tested caused significant antibody-dependent cellular inhibition (ADCI) of parasite growth. Conclusions/Significance: As the majority of subjects vaccinated with MSP2-C1 developed an antibody responses to both forms of MSP2, and that these antibodies mediated ADCI provide further support for MSP2 as a malaria vaccine candidate. However, in view of the reactogenicity of this formulation, further clinical development of MSP2-C1 will require formulation of MSP2 in an alternative adjuvant.
Publisher: PUBLIC LIBRARY SCIENCE
Keywords: PLASMODIUM-FALCIPARUM MALARIA; MEROZOITE SURFACE PROTEIN-2; PAPUA-NEW-GUINEA; ANCHORED MEMBRANE-PROTEINS; IN-VITRO GROWTH; CLINICAL MALARIA; INHIBITORY ANTIBODIES; HUMORAL RESPONSE; ENDEMIC AREA; IMMUNOGENICITY
Research Division(s): Infection And Immunity
Publisher's Version: https://doi.org/10.1371/journal.pone.0024413
Open Access at Publisher's Site: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176224/
Terms of Use/Rights Notice: Copyright This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Documents: journal.pone.0024413.pdf - (0.53MB, application/pdf)

Creation Date: 2011-09-19 12:00:00