Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
- Author(s)
- Keightley, MC; Layton, JE; Hayman, JW; Heath, JK; Lieschke, GJ;
- Details
- Publication Year 2011-08-25,Volume 6,Issue #8,Page -
- Journal Title
- PLOS ONE
- Publication Type
- Journal Article
- Abstract
- Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
- Publisher
- PUBLIC LIBRARY SCIENCE
- Keywords
- TRANSGENIC ZEBRAFISH; MENTAL-RETARDATION; GENE-EXPRESSION; NEURAL CREST; COMPLEX; TRANSCRIPTION; COACTIVATOR; MYC; ASSOCIATION; ERYTHROPOIESIS
- Publisher's Version
- https://doi.org/10.1371/journal.pone.0023845
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2011-08-25 12:00:00