Regulation of memory B-cell survival by the BH3-only protein Puma

Clybouw, C; Fischer, S; Auffredou, MT; Hugues, P; Alexia, C; Bouillet, P; Raphael, M; Leca, G; Strasser, A; Tarlinton, DM; Vazquez, A

Author(s): Clybouw, C; Fischer, S; Auffredou, MT; Hugues, P; Alexia, C; Bouillet, P; Raphael, M; Leca, G; Strasser, A; Tarlinton, DM; Vazquez, A;
Details: Publication Year 2011-10-13,Volume 118,Issue #15,Page 4120-4128
Journal Title: BLOOD
Publication Type: Journal Article
Abstract: Apoptosis is crucial for immune system homeostasis, including selection and survival of long-lived antibody-forming cells and memory cells. The interactions between proapoptotic and pro-survival proteins of the Bcl-2 family are critical for this process. In this report, we show that expression of the proapoptotic BH3-only Bcl-2 family member Puma was selectively up-regulated on in vitro activation with antigens or mitogens of both human and mouse B cells. Puma expression coincided in vivo, with the prosurvival Bcl-2 family member Mcl-1 within the germinal centers and its expression correlates with the germinal center like phenotype of Burkitt lymphoma. Experiments performed in Puma-deficient mice revealed that Puma is essential for apoptosis of mitogen-activated B cells in vitro and for the control of memory B-cell survival. In conclusion, using both human and murine models, our data show that Puma has a major role in the T cell-dependent B-cell immune response. These data demonstrate that Puma is a major regulator of memory B lymphocyte survival and therefore a key molecule in the control of the immune response. (Blood. 2011; 118(15):4120-4128)
Publisher: AMER SOC HEMATOLOGY
Keywords: PROSURVIVAL BCL-2 PROTEINS; ANTIBODY-FORMING-CELLS; GERMINAL CENTER; INDUCED APOPTOSIS; AFFINITY MATURATION; PROGRAMMED DEATH; IMMUNE-RESPONSE; C-MYC; BIM; EXPRESSION
Publisher's Version: https://doi.org/10.1182/blood-2011-04-347096
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2011-10-13 12:00:00