Ndrg1 in development and maintenance of the myelin sheath
- Author(s)
- King, RHM; Chandler, D; Lopaticki, S; Huang, DX; Blake, J; Muddle, JR; Kilpatrick, T; Nourallah, M; Miyata, T; Okuda, T; Carter, KW; Hunter, M; Angelicheva, D; Morahan, G; Kalaydjieva, L;
- Details
- Publication Year 2011-06,Volume 42,Issue #3,Page 368-380
- Journal Title
- NEUROBIOLOGY OF DISEASE
- Publication Type
- Journal Article
- Abstract
- CMT4D disease is a severe autosomal recessive demyelinating neuropathy with extensive axonal loss leading to early disability, caused by mutations in the N-myc downstream regulated gene 1 (NDRG1). NDRG1 is expressed at particularly high levels in the Schwann cell (SC), but its physiological function(s) are unknown. To help with their understanding, we characterise the phenotype of a new mouse model, stretcher (str), with total Ndrg1 deficiency, in comparison with the hypomorphic Ndrg1 knock-out (KO) mouse. While both models display normal initial myelination and a transition to overt pathology between weeks 3 and 5, the markedly more severe str phenotype suggests that even low Ndrg1 expression results in significant phenotype rescue. Neither model replicates fully the features of CMT4D: although axon damage is present, regenerative capacity is unimpaired and the mice do not display the early severe axonal loss typical of the human disease. The widespread large fibre demyelination coincides precisely with the period of rapid growth of the animals and the dramatic (160-500-fold) increase in myelin volume and length in large fibres. This is followed by stabilisation after week 10, while small fibres remain unaffected. Gene expression profiling of str peripheral nerve reveals non-specific secondary changes at weeks 5 and 10 and preliminary data point to normal proteasomal function. Our findings do not support the proposed roles of NDRG1 in growth arrest, terminal differentiation, gene expression regulation and proteasomal degradation. Impaired SC trafficking failing to meet the considerable demands of nerve growth, emerges as the likely pathogenetic mechanism in NDRG1 deficiency. (c) 2011 Elsevier Inc. All rights reserved.
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- DOWNSTREAM-REGULATED GENE-1; SENSORY NEUROPATHY-LOM; MARIE-TOOTH NEUROPATHY; PERIPHERAL-NERVE MYELINATION; PROSTATE-CANCER CELLS; TISSUE GROWTH-FACTOR; LIPOPROTEIN-LIPASE; HEREDITARY MOTOR; SCIATIC-NERVE; DEMYELINATING NEUROPATHY
- Publisher's Version
- https://doi.org/10.1016/j.nbd.2011.01.030
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2011-06-01 12:00:00