The Acquisition of Antigen Cross-Presentation Function by Newly Formed Dendritic Cells
- Author(s)
- Sathe, P; Pooley, J; Vremec, D; Mintern, J; Jin, JO; Wu, L; Kwak, JY; Villadangos, JA; Shortman, K;
- Details
- Publication Year 2011-05-01,Volume 186,Issue #9,Page 5184-5192
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The development of Ag-presenting functions by murine dendritic cells (DCs) of the CD8(+) DC lineage was studied using a Flt-3 ligand stimulated bone-marrow culture system. Although newly formed DCs of this lineage are capable of Ag uptake and efficient presentation to T cells on MHC class II, they initially lack the ability to cross-present exogenous Ags on MHC class I. Cross-presentation capacity is acquired as a subsequent maturation step, promoted by cytokines such as GM-CSF. The development of cross-presentation capacity by the DCs in these cultures may be monitored by the parallel development of DC surface expression of CD103. However, the expression of CD103 and cross-presentation capacity are not always linked; therefore, CD103 is not an essential part of the cross-presentation machinery. These results explain the considerable variability in CD103 expression by CD8(+) DCs as well as the findings that not all DCs of this lineage are capable of cross-presentation. The Journal of Immunology, 2011, 186: 5184-5192.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- BONE-MARROW CULTURES; IN-VIVO; LANGERHANS CELLS; CUTTING EDGE; T-CELLS; CD8(+); SUBSET; EQUIVALENTS; EXPRESSION; GENERATION
- Publisher's Version
- https://doi.org/10.4049/jimmunol.1002683
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2011-05-01 12:00:00