Targeting Antigen to Mouse Dendritic Cells via Clec9A Induces Potent CD4 T Cell Responses Biased toward a Follicular Helper Phenotype
- Author(s)
- Lahoud, MH; Ahmet, F; Kitsoulis, S; Wan, SS; Vremec, D; Lee, CN; Phipson, B; Shi, W; Smyth, GK; Lew, AM; Kato, Y; Mueller, SN; Davey, GM; Heath, WR; Shortman, K; Caminschi, I;
- Details
- Publication Year 2011-07-15,Volume 187,Issue #2,Page 842-850
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Three surface molecules of mouse CD8(+) dendritic cells (DCs), also found on the equivalent human DC subpopulation, were compared as targets for Ab-mediated delivery of Ags, a developing strategy for vaccination. For the production of cytotoxic T cells, DEC-205 and Clec9A, but not Clec12A, were effective targets, although only in the presence of adjuvants. For Ab production, however, Clec9A excelled as a target, even in the absence of adjuvant. Potent humoral immunity was a result of the highly specific expression of Clec9A on DCs, which allowed longer residence of targeting Abs in the bloodstream, prolonged DC Ag presentation, and extended CD4 T cell proliferation, all of which drove highly efficient development of follicular helper T cells. Because Clec9A shows a similar expression pattern on human DCs, it has particular promise as a target for vaccines of human application. The Journal of Immunology, 2011, 187: 842-850.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- C-TYPE LECTIN; IMMUNE-RESPONSES; IN-VIVO; ANTIBODY-RESPONSES; CROSS-PRESENTATION; SUBSET; ACTIVATION; EXPRESSION; EFFICIENT; DEC-205
- Publisher's Version
- https://doi.org/10.4049/jimmunol.1101176
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2011-07-15 12:00:00