Exchange enhanced sensitivity gain for solvent-exchangeable protons in 2D H-1-N-15 heteronuclear correlation spectra acquired with band-selective pulses
- Author(s)
- Yao, SG; Hinds, MG; Murphy, JM; Norton, RS;
- Details
- Publication Year 2011-08,Volume 211,Issue #2,Page 243-247
- Journal Title
- JOURNAL OF MAGNETIC RESONANCE
- Publication Type
- Journal Article
- Abstract
- Conformational or chemical exchange can cause significant sensitivity loss in NMR spectroscopy through resonance broadening for nuclear spins involved in these processes. While this effect may sometimes be alleviated by manipulating experimental conditions such as temperature, pH, and buffers, conditions optimal for all resonances are not always achievable. As a consequence, any means of recovering or minimizing this exchange-induced sensitivity loss is potentially of significant value in regaining information otherwise lost. We report the experimental observation of significant sensitivity gain for nuclear spins undergoing chemical exchange with solvent (water) at exchange rates ca 1-10 s(-1) in H-1-N-15 correlation spectra of proteins acquired with band-selective pulses (the SOFAST-HMQC sequence). (C) 2011 Elsevier Inc. All rights reserved.
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- PROTEIN NMR EXPERIMENTS; CHEMICAL-EXCHANGE; SPECTROSCOPY; RECONSTRUCTION; TEMPERATURE; ACQUISITION; SECONDS; SCHEME
- Publisher's Version
- https://doi.org/10.1016/j.jmr.2011.05.013
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2011-08-01 12:00:00