Murid Herpesvirus-4 Exploits Dendritic Cells to Infect B Cells
- Author(s)
- Gaspar, M; May, JS; Sukla, S; Frederico, B; Gill, MB; Smith, CM; Belz, GT; Stevenson, PG;
- Details
- Publication Year 2011-11,Volume 7,Issue #11,Page e1002346
- Journal Title
- PLOS PATHOGENS
- Publication Type
- Journal Article
- Abstract
- Dendritic cells (DCs) play a central role in initiating immune responses. Some persistent viruses infect DCs and can disrupt their functions in vitro. However, these viruses remain strongly immunogenic in vivo. Thus what role DC infection plays in the pathogenesis of persistent infections is unclear. Here we show that a persistent, B cell-tropic gamma-herpesvirus, Murid Herpesvirus-4 (MuHV-4), infects DCs early after host entry, before it establishes a substantial infection of B cells. DC-specific virus marking by cre-lox recombination revealed that a significant fraction of the virus latent in B cells had passed through a DC, and a virus attenuated for replication in DCs was impaired in B cell colonization. In vitro MuHV-4 dramatically altered the DC cytoskeleton, suggesting that it manipulates DC migration and shape in order to spread. MuHV-4 therefore uses DCs to colonize B cells.
- Publisher
- PUBLIC LIBRARY SCIENCE
- Keywords
- EPSTEIN-BARR-VIRUS; CD8(+) T-CELLS; GAMMA-HERPESVIRUS; IN-VIVO; GAMMAHERPESVIRUS 68; LYMPH-NODES; HUMAN CYTOMEGALOVIRUS; ANTIGEN PRESENTATION; SUBCAPSULAR SINUS; HOST COLONIZATION
- Research Division(s)
- Immunology
- Publisher's Version
- https://doi.org/10.1371/journal.ppat.1002346
- Open Access at Publisher's Site
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213091/- Terms of Use/Rights Notice
- Copyright: © 2011 Gaspar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creation Date: 2011-11-01 12:00:00