A GFP-Actin reporter line to explore microfilament dynamics across the malaria parasite lifecycle
Details
Publication Year 2012-03, Volume 182, Issue #1-2, Page 93-96
Journal Title
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Publication Type
Journal Article
Abstract
Malaria parasite motility relies on an internal parasite actomyosin motor that, when linked to the host cell substrate, propels motile zoites forward. Despite their key role in this process, attempts to visualize actin microfilaments (F-actin) during motility and under native microscopy conditions have not to date been successful. Towards facilitating their visualization we present here a Plasmodium berghei transgenic line in which a green fluorescent protein (GFP)-actin fusion is constitutively expressed through the lifecycle. Focused investigation of the largest motile form, the insect stage ookinete, demonstrates a large cytosolic pool of actin with no obvious F-actin structures. However, following treatment with the actin filament-stabilizing drug Jasplakinolide, we show evidence for concentration of F-actin dynamics in the parasite pellicle and at polar apices. These observations support current models for gliding motility and establish a cellular tool for further exploration of the diverse roles actin is thought to play throughout parasite development. (C) 2011 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Keywords
APICOMPLEXAN PARASITES; TOXOPLASMA-GONDII; PLASMODIUM-FALCIPARUM; ELECTRON-MICROSCOPY; GLIDING MOTILITY; POLYMERIZATION; CYTOSKELETON; FILAMENTS; TURNOVER; INVASION
WEHI Research Division(s)
Infection And Immunity
Rights Notice
Copyright © 2011 Elsevier B.V. All rights reserved.


Creation Date: 2012-03-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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