The development and fate of follicular helper T cells defined by an IL-21 reporter mouse
Details
Publication Year 2012-05, Volume 13, Issue #5, Page 491-498
Journal Title
NATURE IMMUNOLOGY
Publication Type
Journal Article
Abstract
Germinal centers require CD4(+) follicular helper T cells (T-FH cells), whose hallmark is expression of the transcriptional repressor Bcl-6, the chemokine receptor CXCR5 and interleukin 21 (IL-21). To track the development and fate of T-FH cells, we generated an IL-21 reporter mouse by introducing sequence encoding green fluorescent protein (GFP) into the Il21 locus; these mice had expression of IL-21-GFP in CD4(+)CXCR5(+)PD-1(+)T(FH) cells. IL-21-GFP(+) T-FH cells were multifunctional helper cells that coexpressed several cytokines, including interferon-gamma (IFN-gamma), IL-2 and IL-4. T-FH cells proliferated and gave rise to transferrable memory cells with plasticity, which differentiated after recall into conventional effector helper T cells and T-FH cells. Thus, we demonstrated that T-FH cells were not terminally differentiated but instead retained the flexibility to be recruited into other helper T cell subsets and nonlymphoid tissues.
Publisher
NATURE PUBLISHING GROUP
Keywords
GERMINAL CENTER B; IMMUNOGLOBULIN PRODUCTION; TRANSCRIPTION FACTOR; BCL-6 EXPRESSION; TRANSGENIC MICE; EFFECTOR; DIFFERENTIATION; ACTIVATION; GENERATION; INDUCTION
WEHI Research Division(s)
Immunology; Molecular Immunology
Publisher's Version
https://doi.org/10.1038/ni.2261
Rights Notice
© 2012 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.


Creation Date: 2012-05-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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