NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes
Details
Publication Year 2011-12, Volume 8, Issue #12, Page 1037-+
Journal Title
NATURE METHODS
Publication Type
Journal Article
Abstract
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Publisher
NATURE PUBLISHING GROUP
Keywords
EMBRYONIC STEM-CELLS; DIFFERENTIATION; AGGREGATION; MULTIPOTENT; PROTOCOL; BODIES; GENE
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2011-12-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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