NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes

Author(s): Elliott, DA; Braam, SR; Koutsis, K; Ng, ES; Jenny, R; Lagerqvist, EL; Biben, C; Hatzistavrou, T; Hirst, CE; Yu, QC; Skelton, RJP; Oostwaard, DWV; Lim, SM; Khammy, O; Li, XL; Hawes, SM; Davis, RP; Goulburn, AL; Passier, R; Prall, OWJ; Haynes, JM; Pouton, CW; Kaye, DM; Mummery, CL; Elefanty, AG; Stanley, EG;
Details: Publication Year 2011-12,Volume 8,Issue #12,Page 1037-+
Journal Title: NATURE METHODS
Publication Type: Journal Article
Abstract: NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Publisher: NATURE PUBLISHING GROUP
Keywords: EMBRYONIC STEM-CELLS; DIFFERENTIATION; AGGREGATION; MULTIPOTENT; PROTOCOL; BODIES; GENE
Publisher's Version: https://doi.org/10.1038/NMETH.1740
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2011-12-01 12:00:00