Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea

Koepfli, C; Ross, A; Kiniboro, B; Smith, TA; Zimmerman, PA; Siba, P; Mueller, I; Felger, I

Author(s): Koepfli, C; Ross, A; Kiniboro, B; Smith, TA; Zimmerman, PA; Siba, P; Mueller, I; Felger, I;
Details: Publication Year 2011-12,Volume 5,Issue #12,Page e1424
Journal Title: PLOS NEGLECTED TROPICAL DISEASES
Publication Type: Journal Article
Abstract: Plasmodium vivax is highly endemic in the lowlands of Papua New Guinea and accounts for a large proportion of the malaria cases in children less than 5 years of age. We collected 2117 blood samples at 2-monthly intervals from a cohort of 268 children aged 1 to 4.5 years and estimated the diversity and multiplicity of P. vivax infection. All P. vivax clones were genotyped using the merozoite surface protein 1 F3 fragment (msp1F3) and the microsatellite MS16 as molecular markers. High diversity was observed with msp1F3 (H(E) = 88.1%) and MS16 (H(E) = 97.8%). Of the 1162 P. vivax positive samples, 74% harbored multi-clone infections with a mean multiplicity of 2.7 (IQR = 1-3). The multiplicity of P. vivax infection increased slightly with age (P = 0.02), with the strongest increase in very young children. Intensified efforts to control malaria can benefit from knowledge of the diversity and MOI both for assessing the endemic situation and monitoring the effects of interventions.
Publisher: PUBLIC LIBRARY SCIENCE
Keywords: SURFACE PROTEIN-1 GENE; EAST SEPIK PROVINCE; PARASITE POPULATIONS; VACCINE TRIALS; WOSERA AREA; MALARIA; FALCIPARUM; CHILDREN; PATTERNS; TRANSMISSION
Research Division(s): Infection And Immunity
Publisher's Version: https://doi.org/10.1371/journal.pntd.0001424
Open Access at Publisher's Site: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243695/
Terms of Use/Rights Notice: Copyright Koepfli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Documents: journal.pntd.0001424.pdf - (0.28MB, application/pdf)

Creation Date: 2011-12-01 12:00:00