Blood-Stage Plasmodium berghei Infection Generates a Potent, Specific CD8(+) T-Cell Response Despite Residence Largely in Cells Lacking MHC I Processing Machinery
Details
Publication Year 2011-12-15,Volume 204,Issue #12,Page 1989-1996
Journal Title
JOURNAL OF INFECTIOUS DISEASES
Publication Type
Journal Article
Abstract
Murine cerebral malaria is a complex disease caused by Plasmodium berghei ANKA infection. Several cell types, including CD8(+) T cells, are essential effectors of disease. Although the use of transgenic parasites expressing model antigens has revealed the induction of cytotoxic T lymphocytes (CTL) specific for these model antigens, there is no direct evidence for a response to authentic blood-stage parasite antigens, nor any knowledge of its magnitude. Our studies show that there is a dramatic primary parasite-specific CTL response, akin to viral immunity, reaching approximately 30% of splenic CD8(+) T cells, with many producing interferon-gamma and tumor necrosis factor-alpha. These cells express granzyme B and other markers of specific responders, are cytolytic, and respond to a broad array of major histocompatibility complex (MHC) I-restricted epitopes, 5 of which are identified here. Our studies indicate that vigorous CTL responses can be induced to pathogens even when they largely reside in red blood cells, which lack MHC I processing machinery.
Publisher
OXFORD UNIV PRESS INC
Keywords
EXPERIMENTAL CEREBRAL MALARIA; NATURAL-KILLER COMPLEX; DENDRITIC CELLS; PATHOGENESIS; LYMPHOCYTES; RECEPTORS; ANTIGENS; CD4(+)
Research Division(s)
Infection And Immunity
Terms of Use/Rights Notice
© The Author 2011. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.


Creation Date: 2011-12-15 12:00:00
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