Antiviral Activity of Arbidol, a Broad-Spectrum Drug for Use Against Respiratory Viruses, Varies According to Test Conditions
- Author(s)
- Brooks, MJ; Burtseva, EI; Ellery, PJ; Marsh, GA; Lew, AM; Slepushkin, AN; Crowe, SM; Tannock, GA;
- Details
- Publication Year 2012-01,Volume 84,Issue #1,Page 170-181
- Journal Title
- JOURNAL OF MEDICAL VIROLOGY
- Publication Type
- Journal Article
- Abstract
- The therapeutic activity of arbidol was investigated against representatives of seven different virus families. Its 50% median effective concentration (EC50) was 0.22-11.8 mu g/ml (0.41-22 nM). Therapeutic indices of 91 were obtained for type 1 poliovirus and 1.9-8.5 for influenza A and B, human paramyxo-3, avian infectious bronchitis-, and Marek's disease viruses. Arbidol was more inhibitory for influenza A/Aichi/2/68 (H3N2) virus than rimantadine or amantadine (EC50 10 vs. >15 and >31.6 mu g/ml); greater inhibition occurred when end-points were expressed as TCID(50)s. For respiratory syncytial virus (RSV), a reduction in plaque size but not number was observed. However, when the drug was added to infected cultures (>5 mu g/ml), a 3-log reduction in titer occurred. Arbidol did not inhibit directly influenza A/Aichi/2/68 hemagglutinin (HA) or neuraminidase (NA) activity, but inhibition of fusion between the viral envelope and chicken red blood cells occurred when added at 0.1 mu g/ml prior to infection. Arbidol induced changes to viral mRNA synthesis of the PB2, PA, NP, NA, and NS genes in MDCK cultures infected with influenza A/PR/8/34. There was no indirect evidence of enhancement of interferon-alpha by arbidol following infection with A/Aichi/2/68. Arbidol neither reduced lung viral titers nor caused a significant reduction of lung consolidation in BALB/c mice after administration by the oral and intraperitoneal (i.p.) routes and intranasal challenge with influenza A/Aichi/2/68. A small reduction in lung consolidation, but not viral titer, occurred after i.p. administration and subsequent challenge with RSV. The results indicate the potential of arbidol as a broad-spectrum respiratory antiviral drug. J. Med. Virol. 84:170-181, 2012. (C) 2011 Wiley Periodicals, Inc.
- Publisher
- WILEY-BLACKWELL
- Keywords
- INFLUENZA-A VIRUSES; INHIBITOR GS 4071; NEURAMINIDASE INHIBITOR; SYNCYTIAL VIRUS; IN-VITRO; SEASONAL PROPHYLAXIS; INFECTION; INTERFERON; MICE; OSELTAMIVIR
- Research Division(s)
- Immunology
- Publisher's Version
- https://doi.org/10.1002/jmv.22234
- Terms of Use/Rights Notice
- Copyright © 2011 Wiley Periodicals, Inc.
Creation Date: 2012-01-01 12:00:00