Neutrophils sustain effective CD8(+) T-cell responses in the respiratory tract following influenza infection
- Author(s)
- Tate, MD; Brooks, AG; Reading, PC; Mintern, JD;
- Details
- Publication Year 2012-02,Volume 90,Issue #2,Page 197-205
- Journal Title
- IMMUNOLOGY AND CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- Neutrophils have an important role in early host protection during influenza A virus infection. Their ability to modulate the virus-specific adaptive immune response is less clear. Here, we have used a mouse model to examine the impact of neutrophils on CD8(+) T-cell responses during influenza virus infection. CD8(+) T-cell priming, expansion, migration, cytokine secretion and cytotoxic capacity were investigated in the virus-infected airways and secondary lymphoid organs. To do this, we utilised a Ly6G-specific monoclonal antibody (mAb; 1A8) that specifically depletes neutrophils in vivo. Neutrophil depletion early after infection with influenza virus strain HKx31 (H3N2) did not alter influenza virus-derived antigen presentation or naive CD8(+) T-cell expansion in the secondary lymphoid organs. Trafficking of virus-specific CD8(+) T cells into the infected pulmonary airways was also unaltered. Instead, early neutropenia reduced both the overall magnitude of influenza virus-specific CD8(+) T cells, together with impaired cytokine production and cytotoxic effector function. Therefore, neutrophils are important participants in anti-viral mechanisms that sustain effective CD8(+) T-cell responses in the respiratory tract of influenza virus-infected mice. Immunology and Cell Biology (2012) 90, 197-205; doi:10.1038/icb.2011.26; published online 12 April 2011
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- SIMPLEX-VIRUS TYPE-1; ANTIGEN PRESENTATION; DENDRITIC CELLS; IN-VIVO; POLYMORPHONUCLEAR NEUTROPHILS; MONOCLONAL-ANTIBODY; PULMONARY INFECTION; RESTRICTED ANTIGEN; MICE; EXPRESSION
- Research Division(s)
- Immunology
- Publisher's Version
- https://doi.org/10.1038/icb.2011.26
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- © 2013 Australasian Society for Immunology
Creation Date: 2012-02-01 12:00:00