Immunophenotypic changes induced on human HL60 leukaemia cells by 1 alpha,25-dihydroxyvitamin D-3 and 12-O-tetradecanoyl phorbol-13-acetate

Author(s): White, SL; Belov, L; Barber, N; Hodgkin, PD; Christopherson, RI;
Details: Publication Year 2005-10,Volume 29,Issue #10,Page 1141-1151
Journal Title: LEUKEMIA RESEARCH
Publication Type: Journal Article
Abstract: 1 alpha,25-Dihydroxyvitamin D-3 (1,25D(3)) induces HL60 cells to acquire a monocyte-like phenotype, while cells treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA) resemble macrophages. Using a microarray of 82 CD antibodies, 24 cluster of differentiation (CD) antigens were detected on HL60 cells. 1,25D(3) induced the following antigens in decreasing order of the change: CD14, CD11c, CD11b, CD54, CD86, CD38 and CD66c, with repression of CD117, CD71, CD95, CD45 and CD64. TPA induced the following antigens in decreasing order of the change: CD11c, CD9, CD11b, CD54, CD38, CD45RO and CD66c, with repression of CD4, CD117, CD95, CD71 and CD64. The results presented provide a basis for monitoring differentiation therapy of myeloid leukaemias in patients. (c) 2005 Elsevier Ltd. All rights reserved.
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Keywords: ACUTE MYELOID-LEUKEMIA; TRANS-RETINOIC ACID; PROTEIN-KINASE-C; HL-60 CELLS; MONOCYTIC DIFFERENTIATION; 1,25-DIHYDROXYVITAMIN D-3; GENE-EXPRESSION; TERMINAL DIFFERENTIATION; ANTIGEN-EXPRESSION; FLOW-CYTOMETRY
Publisher's Version: https://doi.org/10.1016/j.leukres.2005.02.012
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2005-10-01 12:00:00