Decision criteria for resolving isotype switching conflicts by B cells

Author(s): Deenick, EK; Hasbold, J; Hodgkin, PD;
Details: Publication Year 2005-10,Volume 35,Issue #10,Page 2949-2955
Journal Title: EUROPEAN JOURNAL OF IMMUNOLOGY
Publication Type: Journal Article
Abstract: Isotype switching by B cells is highly regulated by a group of cytokines including IL-4, IFN-gamma and TGF-beta. A B cell can only express one isotype at a time; however during an, immune response it may be exposed to combinations of stimuli that provide it with conflicting switching instructions. To determine how such cytokine-induced isotype switch conflicts would be resolved, the responses of B cells exposed to multiple cytokines were investigated. To eliminate complications arising from simultaneous effects of switching cytokines on proliferation, division number was used as a reference framework to monitor switching rate. The results show a clear hierarchy in which IFN-gamma is dominant over IL-4, and both IL-4 and IFN-gamma are dominant over TGF-beta. These studies reveal how B cells possess a set of logical decision criteria for dealing with pathogens that invoke a range of different stimuli.
Publisher: WILEY-V C H VERLAG GMBH
Keywords: STIMULATORY FACTOR-I; INTERFERON-GAMMA; IGA PRODUCTION; CD40 LIGAND; IFN-GAMMA; DIVISION; DIFFERENTIATION; IGG1; LIPOPOLYSACCHARIDE; EXPRESSION
Publisher's Version: https://doi.org/10.1002/eji.200425719
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2005-10-01 12:00:00