NF-kappa B couples protein kinase B/Akt signaling to distinct survival pathways and the regulation of lymphocyte homeostasis in vivo
- Author(s)
- Jones, RG; Saibil, SD; Pun, JM; Elford, AR; Bonnard, M; Pellegrini, M; Arya, S; Parsons, ME; Krawczyk, CM; Gerondakis, S; Yeh, WC; Woodgett, JR; Boothby, MR; Ohashi, PS;
- Details
- Publication Year 2005-09-15,Volume 175,Issue #6,Page 3790-3799
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Protein kinase B (PKB alpha/Akt1) a PI3K-dependent serine-threonine kinase, promotes T cell viability in response to many stimuli and regulates homeostasis and autoimmune disease in vivo. To dissect the mechanisms by which PKB inhibits apoptosis, we have examined the pathways downstream of PKB that promote survival after cytokine withdrawal vs Fas-mediated death. Our studies show that PKB-mediated survival after cytokine withdrawal is independent of protein synthesis and the induction of NF-kappa B. In contrast, PKB requires de novo gene transcription by NF-kappa B to block apoptosis triggered by the Fas death receptor. Using gene-deficient and transgenic mouse models, we establish that NF-kappa B1, and not c-Rel, is the critical signaling molecule downstream of the PI3K-PTEN-PKB signaling axis that regulates lymphocyte homeostasis.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- AUTOIMMUNE LYMPHOPROLIFERATIVE SYNDROME; FAS-MEDIATED APOPTOSIS; T-CELL SURVIVAL; PHOSPHATIDYLINOSITOL 3-KINASE; DEPENDENT PHOSPHORYLATION; IMMUNE-RESPONSES; RELA/P65 SUBUNIT; C-FLIP; ACTIVATION; DEATH
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- Refer to copyright notice on published article.
Creation Date: 2005-09-15 12:00:00