The development of functional B lymphocytes in conditional PU.1 knock-out mice
Publication Year 2005-09-15, Volume 106, Issue #6, Page 2083-2090
- Journal Title
- Publication Type
- Journal Article
- An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage-positive cells in the fetal liver and death between E18.5 and birth; however, specific deletion of PU.1 in the B lineage had no effect on B-cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon B-cell receptor (BCR) cross-linking; however, this was partially rescued by interieukin-4 (IL-4). These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the pre-B stage.
- AMER SOC HEMATOLOGY
- THYMIC STROMAL LYMPHOPOIETIN; SPI-B; INTERLEUKIN-7 RECEPTOR; ETS PROTEIN; HEMATOPOIETIC PROGENITORS; REGULATES EXPRESSION; LYMPHOID PROGENITORS; ENHANCER ACTIVITY; IMMUNE-RESPONSE; MATURE B
- Publisher's Version
- Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2005-09-15 12:00:00Last Modified: 0001-01-01 12:00:00