The development of functional B lymphocytes in conditional PU.1 knock-out mice
Details
Publication Year 2005-09-15, Volume 106, Issue #6, Page 2083-2090
Journal Title
BLOOD
Publication Type
Journal Article
Abstract
An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage-positive cells in the fetal liver and death between E18.5 and birth; however, specific deletion of PU.1 in the B lineage had no effect on B-cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon B-cell receptor (BCR) cross-linking; however, this was partially rescued by interieukin-4 (IL-4). These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the pre-B stage.
Publisher
AMER SOC HEMATOLOGY
Keywords
THYMIC STROMAL LYMPHOPOIETIN; SPI-B; INTERLEUKIN-7 RECEPTOR; ETS PROTEIN; HEMATOPOIETIC PROGENITORS; REGULATES EXPRESSION; LYMPHOID PROGENITORS; ENHANCER ACTIVITY; IMMUNE-RESPONSE; MATURE B
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2005-09-15 12:00:00
Last Modified: 0001-01-01 12:00:00
An error has occurred. This application may no longer respond until reloaded. Reload 🗙