Cutting edge: Generation of splenic CD8(+) and CD8(-) dendritic cell equivalents in fms-like tyrosine kinase 3 ligand bone marrow cultures
Details
Publication Year 2005-06-01, Volume 174, Issue #11, Page 6592-6597
Journal Title
JOURNAL OF IMMUNOLOGY
Publication Type
Journal Article
Abstract
We demonstrate that functional and phenotypic equivalents of mouse splenic CD8(+) and CD8(-) conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RA(high) plasmacytoid DC, two distinct CD24(high) and CD11b(high) cDC subsets were present, and these subsets showed equivalent properties to splenic CD8(+) and CD8(-) cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-alpha; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors, 4) production of IL-12 p40/70, IFN-alpha, MIP-1 alpha, and RANTES in response to TLR ligands; 5) expression of cystatin C, and 6) cross-presentation of exogenous Ag to CD8 T cells. Furthermore, despite lacking surface CD8 expression, the CD24(high) subset contained CD8 mRNA and up-regulated surface expression when transferred into mice. This culture system allows access to bona fide counterparts of the splenic DC subsets.
Publisher
AMER ASSOC IMMUNOLOGISTS
Keywords
COLONY-STIMULATING FACTOR; FLT3 LIGAND; DIFFERENTIAL PRODUCTION; ANTIGEN PRESENTATION; SOLUBLE-ANTIGEN; IFN-ALPHA; MOUSE; SUBSETS; SPLEEN; SUBTYPES
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Creation Date: 2005-06-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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