Cutting edge: Generation of splenic CD8(+) and CD8(-) dendritic cell equivalents in fms-like tyrosine kinase 3 ligand bone marrow cultures
- Author(s)
- Naik, SH; Proietto, AI; Wilson, NS; Dakic, A; Schnorrer, P; Fuchsberger, M; Lahoud, MH; O'Keeffe, M; Shao, QX; Chen, WF; Villadangos, JA; Shortman, K; Wu, L;
- Details
- Publication Year 2005-06-01,Volume 174,Issue #11,Page 6592-6597
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- We demonstrate that functional and phenotypic equivalents of mouse splenic CD8(+) and CD8(-) conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RA(high) plasmacytoid DC, two distinct CD24(high) and CD11b(high) cDC subsets were present, and these subsets showed equivalent properties to splenic CD8(+) and CD8(-) cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-alpha; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors, 4) production of IL-12 p40/70, IFN-alpha, MIP-1 alpha, and RANTES in response to TLR ligands; 5) expression of cystatin C, and 6) cross-presentation of exogenous Ag to CD8 T cells. Furthermore, despite lacking surface CD8 expression, the CD24(high) subset contained CD8 mRNA and up-regulated surface expression when transferred into mice. This culture system allows access to bona fide counterparts of the splenic DC subsets.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- COLONY-STIMULATING FACTOR; FLT3 LIGAND; DIFFERENTIAL PRODUCTION; ANTIGEN PRESENTATION; SOLUBLE-ANTIGEN; IFN-ALPHA; MOUSE; SUBSETS; SPLEEN; SUBTYPES
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Creation Date: 2005-06-01 12:00:00