Relaxin and prostaglandin E-2 regulate interleukin 11 during human endometrial stromal cell decidualization

Dimitriadis, E; Stoikos, C; Baca, M; Fairlie, WD; McCoubrie, JE; Salamonsen, LA

Author(s): Dimitriadis, E; Stoikos, C; Baca, M; Fairlie, WD; McCoubrie, JE; Salamonsen, LA;
Details: Publication Year 2005-06,Volume 90,Issue #6,Page 3458-3465
Journal Title: JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
Publication Type: Journal Article
Abstract: Decidualization of endometrial stromal cells and IL-11 signaling are essential for embryo implantation in the mouse. We investigated the effects of relaxin ( RLX) and prostaglandin E-2 (PGE(2)) on IL-11 secretion by human endometrial stromal cells (HESC) and during cAMP or medroxyprogesterone acetate (P)-induced decidualization. cAMP-decidualized HESC secreted high levels of IL-11. RLX, cAMP, or PGE(2) increased IL-11 mRNA and IL-11 secretion, with maximal response to RLX and cAMP. Addition of the cAMP/protein kinase A inhibitor Rp-adenosine-3,5-cyclic-monophosphorothioate to either RLX- or PGE(2)-treated cells decreased IL-11 secretion. Indomethacin treatment decreased IL-11 secretion, which was largely restored by cotreatment with PGE(2) or RLX. Cotreatment of HESC with RLX, PGE(2), or cAMP and estrogen plus P down-regulated IL-11 mRNA and IL-11 secretion at 24 h, before secretion of prolactin ( decidualization marker). Addition of W147AIL-11 (IL-11 signaling inhibitor) reduced prolactin secretion stimulated by RLX or PGE(2) and estrogen plus P. This is the first demonstration that cAMP-decidualized HESC secrete IL-11 and that IL-11mRNAand IL-11 secretion are regulated by RLX and PGE(2), partly via a cAMP/protein kinase A-dependent pathway. Blocking IL-11 signaling reduced RLX + P- or PGE(2) + P- induced decidualization, suggesting that RLX and PGE(2) act via IL-11. This is important in understanding implantation and regulation of fertility.
Publisher: ENDOCRINE SOC
Keywords: PROTEIN-KINASE-A; IN-VITRO; MENSTRUAL-CYCLE; EXPRESSION; RECEPTOR; PROLACTIN; GENE; DIFFERENTIATION; IMPLANTATION; LOCALIZATION
Publisher's Version: https://doi.org/10.1210/jc.2004-1014
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2005-06-01 12:00:00