The gene trap resource: A treasure trove for hemopoiesis research
- Details
- Publication Year 2005-08,Volume 33,Issue #8,Page 845-856
- Journal Title
- EXPERIMENTAL HEMATOLOGY
- Publication Type
- Journal Article
- Abstract
- The laboratory mouse is an invaluable tool for functional gene discovery because of its genetic malleability and a biological similarity to human systems that facilitates identification of human models of disease. A number of mutagenic technologies are being used to elucidate gene function in the mouse. Gene trapping is an insertional mutagenesis strategy that is being undertaken by multiple research groups, both academic and private, in an effort to introduce mutations across the mouse genome. Large-scale, publicly funded gene trap programs have been initiated in several countries with the International Gene Trap Consortium coordinating certain efforts and resources. We outline the methodology of mammalian gene trapping and how it can be used to identify genes expressed in both primitive and definitive blood cells and to discover hemopoietic regulator genes. Mouse mutants with hematopoietic phenotypes derived using gene trapping are described. The efforts of the large-scale gene trapping consortia have now led to the availability of libraries of mutagenized ES cell clones. The identity of the trapped locus in each of these clones can be identified by sequence-based searching via the world wide web. This resource provides an extraordinary tool for all researchers wishing to use mouse genetics to understand gene function. (c) 2005 International Society for Experimental Hematology. Published by Elsevier Inc.
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- EMBRYONIC STEM-CELLS; DEVELOPMENTALLY-REGULATED GENES; PUROMYCIN-SENSITIVE AMINOPEPTIDASE; IN-VITRO DIFFERENTIATION; MICROTUBULE-ASSOCIATED PROTEIN; ZINC-FINGER PROTEIN; FUNCTIONAL-ANALYSIS; MOUSE DEVELOPMENT; MICE LACKING; BINDING PROTEIN
- Publisher's Version
- https://doi.org/10.1016/j.exphem.2005.03.016
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2005-08-01 12:00:00