Molecular cloning and characterization of pig, cow and sheep MAdCAM-1 cDNA and the demonstration of cross-reactive epitopes amongst mammalian homologues
- Author(s)
- Tachedjian, M; Yu, M; Lew, AM; Rockman, S; Boyle, JS; Andrew, ME; Wang, L;
- Details
- Publication Year 2006-05,Volume 67,Issue #5,Page 419-426
- Journal Title
- TISSUE ANTIGENS
- Publication Type
- Journal Article
- Abstract
- Full-length cDNA clones for the pig, cow and sheep mucosal addressin cellular adhesion molecule (MAdCAM)-1 homologues were isolated from Peyer's patches by a combination of reverse transcription (RT)-polymerase chain reaction and 5' and 3' RACE strategies. Degenerate primers based on conserved amino acid (aa) sequences within the N-terminal immunoglobulin (Ig)-like domains of the human and rodent MAdCAM-1 molecules were used for initial sequencing of the Ig-like domains. MAdCAM-1 transcripts of 1425 bp, 1525 bp and 1510 bp obtained for the pig, cow and sheep contained an open-reading frame for proteins of 390, 424 and 418 aa, respectively. The pig and ruminant MAdCAM-1 had two N-terminal Ig-like domains, a mucin-like region and a third Ig-like domain found in rodent but not human MAdCAM-1. Antibodies raised against bacterially expressed N-terminal Ig-like domains of pig, human and sheep MAdCAM-1 demonstrated the existence of cross-reactive epitopes, raising the possibility of producing monoclonal antibodies which can be used as multi-species MAdCAM-1-targeting reagent for the development of mucosal vaccines.
- Publisher
- BLACKWELL PUBLISHING
- Keywords
- CELL-ADHESION MOLECULE-1; INTEGRIN RECOGNITION; ADDRESSIN MADCAM-1; BINDING; IMMUNOGLOBULIN; DOMAINS; ALPHA(4)BETA(7); SUBUNIT; REVEALS; TISSUE
- Publisher's Version
- https://doi.org/10.1111/j.1399-0039.2006.00587.x
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2006-05-01 12:00:00