Socs2 and Elf5 mediate prolactin-induced mammary gland development

Harris, J; Stanford, PM; Sutherland, K; Oakes, SR; Naylor, MJ; Robertson, FG; Blazek, KD; Kazlauskas, M; Hilton, HN; Wittlin, S; Alexander, WS; Lindeman, GJ; Visvader, JE; Ormandy, CJ

Author(s): Harris, J; Stanford, PM; Sutherland, K; Oakes, SR; Naylor, MJ; Robertson, FG; Blazek, KD; Kazlauskas, M; Hilton, HN; Wittlin, S; Alexander, WS; Lindeman, GJ; Visvader, JE; Ormandy, CJ;
Details: Publication Year 2006-05,Volume 20,Issue #5,Page 1177-1187
Journal Title: MOLECULAR ENDOCRINOLOGY
Publication Type: Journal Article
Abstract: The proliferative phase of mammary alveolar morphogenesis is initiated during early pregnancy by rising levels of serum prolactin and progesterone, establishing a program of gene expression that is ultimately responsible for the development of the lobuloalveoli and the onset of lactation. To explore this largely unknown genetic program, we constructed transcript profiles derived from transplanted mammary glands formed by recombination of prolactin receptor (Prlr) knockout or wildtype mammary epithelium with wild-type mammary stroma. Comparison with profiles derived from prolactin-treated Scp2 mammary epithelial cells produced a small set of commonly prolactin-regulated genes that included the negative regulator of cytokine signaling, Socs2 (suppressor of cytokine signaling 2), and the ets transcription factor, E74-like factor 5 (Elf5). Homozygous null mutation of Socs2 rescued the failure of lactation and reduction of mammary signal transducer and activator of transcription 5 phosphorylation that characterizes Prlr heterozygous mice, demonstrating that mammary Socs2 is a key regulator of the prolactin-signaling pathway. Reexpression of Elf5 in Prlr nullizygous mammary epithelium restored lobuloalveolar development and milk production, demonstrating that Elf5 is a transcription factor capable of substituting for prolactin signaling. Thus, Socs2 and Elf5 are key members of the set of prolactin-regulated genes that mediate prolactin-driven mammary development.
Publisher: ENDOCRINE SOC
Keywords: RECEPTOR-DEFICIENT MICE; HUMAN BREAST-CANCER; TRANSCRIPTION FACTORS; GROWTH-HORMONE; MESSENGER-RNA; KNOCKOUT MICE; ETS GENES; MOUSE; EXPRESSION; CARCINOMA
Publisher's Version: https://doi.org/10.1210/me.2005-0473
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2006-05-01 12:00:00