The proliferative response of CD4 T cells to steady-state CD8(+) dendritic cells is restricted by post-activation death
- Author(s)
- Rizzitelli, A; Hawkins, E; Todd, H; Hodgkin, PD; Shortman, K;
- Details
- Publication Year 2006-03,Volume 18,Issue #3,Page 415-423
- Journal Title
- INTERNATIONAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- CD8(+) splenic dendritic cells (DCs) from steady-state mice are less effective than the CD8(-) DC subset in their capacity to stimulate CD4 T cell proliferation in culture. However, we found that the two DC subtypes were equally potent at activating CD4 T cells, based on up-regulation of CD69 and CD25 expression. Also, we found no difference in the rate of T cell death prior to entry into the first division. We then tracked carboxyfluorescein diacetate succinimidyl ester-labeled T cells and employed a quantitative model to assess in detail the CD4 T cell expansion process in response to stimulation with CD8(+) or with CD8(-) DCs. The time required for most T cells to replicate their DNA prior to the first division was similar in both DC cultures. However, progression of the CD4 T cell population through subsequent divisions was reduced in CD8(+) DCs compared with CD8(-) DC culture. This was associated with an increased loss of viable T cells at each division. Post-activation, division-associated T cell death is therefore a major factor in the reduced response of CD4 T cells to CD8(+) DCs.
- Publisher
- OXFORD UNIV PRESS
- Keywords
- IN-VIVO; MOUSE THYMUS; EXPRESSION; TOLERANCE; SUBPOPULATION; SELECTION; CALCULUS; IMMUNITY; SUBSETS; SPLEEN
- Publisher's Version
- https://doi.org/10.1093/intimm/dxh382
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2006-03-01 12:00:00