Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish

Hogan, BM; Layton, JE; Pyati, UJ; Nutt, SL; Hayman, JW; Varma, S; Heath, JK; Kimelman, D; Lieschke, GJ

Author(s): Hogan, BM; Layton, JE; Pyati, UJ; Nutt, SL; Hayman, JW; Varma, S; Heath, JK; Kimelman, D; Lieschke, GJ;
Details: Publication Year 2006-03-07,Volume 16,Issue #5,Page 506-511
Journal Title: CURRENT BIOLOGY
Publication Type: Journal Article
Abstract: In the zebrafish embryo, primitive hematopoiesis initiates in two spatially distinct regions. Rostrally, the cells of the anterior lateral plate mesoderm (ALPM) give rise exclusively to cells of the myeloid lineage in a pu.1-dependent manner [1-5]. Caudally, in the posterior lateral plate mesoderm (PLPM), the expression of gata1 defines a precursor pool that gives rise predominantly to the embryonic erythrocytes [6]. The transcription factor scl acts upstream of both gata1 and pu.1 in these precursor pools, activating a series of conserved transcription factors that cell-autonomously specify either myeloid or erythroid fates [1, 4, 7, 8]. However, the mechanisms underlying the spatial separation of the hematopoietic precursor pools and the induction of differential gene expression within these pools are not well understood. We show here that the Bmp receptor lost-a-fin/alk8 is required for rostral pu.1 expression and myelopoiesis, identifying an early genetic event that distinguishes between the induction of anterior and posterior hematopolesis. Introducing a constitutively active version of the Alk8 receptor led to increased pu.1 expression, but the role of alk8 was independent of the scl-dependent cell-fate pathway. Furthermore, the role of Alk8 in myelopoiesis was genetically separable from its earlier role in dorsalventral embryonic patterning.
Publisher: CELL PRESS
Keywords: SERINE/THREONINE KINASE RECEPTOR; NEURAL CREST CELLS; DIFFERENTIATION; EXPRESSION; EMBRYO; CLOCHE; BLOOD; PU.1; GENE; SCL
Publisher's Version: https://doi.org/10.1016/j.cub.2006.01.047
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2006-03-07 12:00:00