Glycoprotein G deletion mutants of equine herpesvirus 1 (EHV1; equine abortion virus) and EHV4 (equine rhinopneumonitis virus)
Details
Publication Year 2005-12,Volume 150,Issue #12,Page 2583-2592
Journal Title
ARCHIVES OF VIROLOGY
Publication Type
Journal Article
Abstract
Glycoprotein G (gG) deletion mutants of EHV1 and EHV4, designated EHV1 Delta gG and EHV4 Delta gG, were constructed. The growth characteristics of the EHV1 Delta gG mutants were similar to the parent virus. All of the EHV4 Delta gG mutants grew more slowly in cell culture and produced plaques of different morphology including smaller size. The yields of both gG deletion mutant viruses in cell culture were similar to the parent viruses. Sequencing of the genes flanking gG, Southern blot, PCR and western blot analyses of the mutant viruses demonstrated that the deletions were as expected, except for EHV4 Delta gG mutants, which in addition to deletion of gG contained unexpected deletions in the adjacent down stream gene ORF 71 (glycoprotein 2). Antisera to EHV1 Delta gG and EHV4 Delta gG neutralised the respective mutant and the parent viruses to the same titre and these antisera could be distinguished from antisera to the wild type viruses in a gG antibody detection ELISA. The mutant viruses may be useful as vaccine candidates and the deletion of gG may act as a marker to distinguish vaccinated from the naturally infected horses.
Publisher
SPRINGER WIEN
Keywords
ASININE HERPESVIRUS-3; NUCLEOTIDE-SEQUENCE; DNA-SEQUENCE; TYPE-1; PROTEINS; OUTBREAK; ELISA
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Creation Date: 2005-12-01 12:00:00
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